3.2.4 [pH]o imaging in proton releasing cells by an ion image sensor-based chemical microscopy

Event
14th International Meeting on Chemical Sensors - IMCS 2012
2012-05-20 - 2012-05-23
Nürnberg/Nuremberg, Germany
Chapter
3.2 Biosensors III (cell based)
Author(s)
T. Sakurai - Electronics-Inspired Interdisciplinary Research Institute, Toyohashi University of Technology (Japan), H. Taki, K. Okumura, K. Sawada - Department of Electrical and Electronic Information Engineering, Toyohashi University of Technology; Susumu Terakawa, JST CREST, Tokyo (Japan), Y. Fukushi - Medical Photonics Researce Center, Hamamatsu University, School of Medicine (Japan)
Pages
273 - 274
DOI
10.5162/IMCS2012/3.2.4
ISBN
978-3-9813484-2-2
Price
free

Abstract

To examine the functions of proton release from the cells, we measured the extracellular pH ([pH]o) by using an ion image sensor made of a 2 dimensional array of potential sensitive pixels. We recorded the changes in [pH] o of cells such as osteoclasts, gastric glands and hippocampal slices. After stimulation of cells and/or tissues, [pH]o was decreased, and the degree of the pH changes was dependent on the doses of stimulants. We established a label free microscopic assessment of 2Ddistributions of biologically interesting substances by the present chemical sensing technique. The [pH] o imaging by chemical microscope promises the usefulness in the medical fields for the analysis of diseases such as osteoporosis, gastric ulcer, and stroke.

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