O3.1 - Fluorescent Nanosensors for Monitoring Ions in Biosamples
- SENSOR+TEST Conferences 2009
2009-05-26 - 2009-05-28
Congress Center Nürnberg
- Proceedings OPTO 2009 & IRS² 2009
- OPTO 3 - Applications and Devices
- T. Doussineau, G. Mohr, A. Schulz, S. Trupp, A. Moro, P. Cywinski, S. Koersten, B. Bussemer, A. Lapresta-Fernandez - Friedrich-Schiller-University Jena, Jena, Germany
- 71 - 74
Fluorescent nanoparticles have recently found increasing interest in medical and biological research. Significant improvements have already been achieved for the detection of ions and biomolecules in living cells, tissues and microorganisms in comparison to conventional fluorescent probes. In a general point of view, dyes embedded in a polymeric matrix show a better photostability thanks to the spatial constraint of the dye molecule together with the limited access of dioxygen within the matrix. Additionally, the system dye@matrix exhibits a higher brightness due to the high concentration of the emittiing molecules within a reduced space and this without the limitations observed in solution like the aggregation phenomenon. In biological medium, the embedding of the dye reduces its toxicity and non-specific interactions with biomolecules like proteins that can cause misinterpretation in the changes of the optical signal. Finally, it is possible to immobilise not only indicator dyes but also inert reference dyes in the particles. This allows ratiometric measurements for reliable and continuous analyte monitoring.
Based on these principles, we report here results on the development of nanosensors for H+ and Cl- that constitute promising tools for bioanalysis. Advantages and limitations of polymer matrices will be discussed.