5.1.2 - Multiplexed analysis of extracellular vesicles from single cells in an array with opposing microwells
- Event
- EUROSENSORS 2026
2026-09-06 - 2026-09-09
Zurich - Band
- Lectures
- Chapter
- Microfluidic Devices, Lab on a Chip, µTAS, Organ on Chip Systems
- Author(s)
- L. R. Stöcklin, E. Kortus, P. S. Dittrich - ETH Zürich,Basel (Switzerland)
- Pages
- 160 - 161
- DOI
- 10.5162/eurosensors2026/5.1.2
- ISBN
- 978-3-910600-12-6
- Price
- free
Abstract
Single-cell analysis can help to resolve the heterogeneous and complex biogenesis of extracellular vesicles (EVs), thereby improving our understanding of EVs’ role in signalling and cell-cell communication. Here, we introduce a method to detect up to five membrane proteins in EVs secreted from single cells. Two microwell arrays, one for single-cell trapping and another one for capturing fluorescently barcoded beads pre-functionalized with EV-capture antibodies, are aligned during incubation. Then, membrane proteins of bead-bound EVs are analysed by immunostaining for four breast-derived cell lines.