3.4 - Detection of the metabolic activity of cells by differential measurements based on a single light-adressable potentiometric sensor chip
- 11. Dresdner Sensor-Symposium 2013
2013-12-09 - 2013-12-11
Dreikönigskirche - Haus der Kirche Dresden
- S. Takenaga, C. Herrera, C. Werner, M. Biselli, T. Schnitzler, M. Schöning, P. Öhlschläger, T. Wagner - FH Aachen, Jülich/D
- 63 - 67
The metabolic activity of Chinese hamster ovary (CHO) cells was successfully observed using a lightaddressable potentiometric sensor (LAPS). The dependency of the different glucose concentrations (17-200 mM) follows a Michaelis-Menten kinetics like trajectory. Moreover, a good adhesion of CHO cells on the Ta2O5 surface of the sensor chip was observed, and the cells could culture under normal conditions. These results demonstrate the detection of the metabolic response from cells by means of a LAPS-based set-up.