SP 2.2.3 - Isolation and culturing of urinary bacteria for VOC-based rapid diagnostics

Event

EUROSENSORS 2026
2026-09-06 - 2026-09-09
Zurich

Band

Lectures

Chapter

Antimicrobial Resistance Diagnostics

Author(s)

A. Vescovi, G. Vasku, E. Wetter Slack - ETH Zürich,Zürich (Switzerland)

Pages

305 - 306

DOI

10.5162/eurosensors2026/SP2.2.3

ISBN

978-3-910600-12-6

Price

free

Abstract

Rapid alternatives to traditional 2 - 4 day urine culture are urgently needed to improve patient recovery and combat antimicrobial resistance. Volatile organic compound (VOC) sensing offers a powerful, non-invasive route to bacterial identification, but establishing reproducible metabolic markers requires a workflow that accounts for both pathogen diversity and variable growth dynamics. Using expanded urine culture, we isolated bacteria from 80 patient samples, revealing 35 distinct genera and pronounced inter-individual compositional variability. Growth kinetics of 75 E. coli strains were benchmarked under urinary-like conditions, revealing significant lag-time variation that motivates rigorous standardization for reproducible VOC profiling.