PT3 - MIP nanoparticles in diagnostics and bioimaging
- 17th International Meeting on Chemical Sensors - IMCS 2018
2018-07-15 - 2018-07-19
- Plenary Talk
- S. Piletsky - College of Science and Engineering, University of Leicester (Great Britain)
- 59 - 59
Two years ago we have made a major breakthrough in MIP technology developing solid-phase approach for preparation of soluble molecularly imprinted nanoparticles (nanoMIPs) with exquisite affinity and selectivity for their templates1. The success came from combining controlled radical polymerisation2 with an affinity separation step performed on surface-immobilised template3. This approach represents the state-of-the-art in nanoMIP synthesis: not only are soluble particles with defined size (20-200 nm) and a narrow size distribution produced in one hour, they possess subnanomolar dissociation constants for their respective targets, they can be easily functionalised with fluorescent, electrochemical or magnetic labels, and the immobilised template can be re-used. High affinity nanoMIPs were made for small molecules, proteins, membrane proteins and virus particles.