BS1.2 - Phosphorescence based oxygen sensors - essential tools for cell biology and life science research
- 17th International Meeting on Chemical Sensors - IMCS 2018
2018-07-15 - 2018-07-19
- Biosensors 1
- D. Papkovsky - School of Biochemistry and Cell Biology, University College Cork, Cork (Ireland)
- 71 - 72
Various in vitro, ex-vivo and in vivo cell and tissue models are currently used in life science research, however for many of them control of sample oxygenation and cellular O2 levels is inadequate. Since O2 is a key parameter and informative biomarker of cellular function, knowledge of actual O2 levels in different compartments of biological samples and implementation of reliable in situ control of cellular O2 are of paramount importance. Phosphorescence based O2 sensors provide such capabilities and enable many important bioanalytical tasks with complex biological samples. Here I describe the spectrum of O2 sensing platforms developed in recent years by us and other research groups. These systems operate with solid-state sensors, soluble probes and imaging nanosensors, in conjunction with portable handheld instruments, commercial plate readers and sophisticated live cell imaging detection platforms. Analytical performance and practical uses of these sensor systems will be demonstrated in examples of physiological studies performed with simple 2D cell models, more complex multicellular spheroids, hetero-cellular organoids and cultured tissue slices. Integration of these tools in the current paradigm of life science research will also be discussed.