OS2.2 - M13 Bacteriophage Functionalized Silver Nanowire Surface-Enhanced Raman Scattering Sensor
- 17th International Meeting on Chemical Sensors - IMCS 2018
2018-07-15 - 2018-07-19
- Optical Sensors 2
- H. Jung, E. Koh, S. Park, D. Kim - Advanced Functional Thin Films Department, Korea Institutes of Materials Science (KIMS), Changwon, Gyeongnam (South Korea), J. Oh - Department of Nano Fusion Technology, Pusan National University (PNU), Busan (South Korea)
- 120 - 121
A surface-enhanced Raman scattering (SERS) sensor comprising silver nanowires (AgNWs) and genetically engineered M13 Bacteriophages (BPWHW) was fabricated onto a glass-fiber filter paper (GFFP) membrane. AgNWs stacked on GFFP formed high density of SERS-active hot spots and the surface-coated BPWHW functioned as bioreceptor for selective pesticide detection. The Raman signal enhancement and the selective SERS detection of pesticides were investigated compared with wildtype M13 bacteriophage-decorated AgNWs (BPWT/AgNW) and undecorated AgNWs (AgNW). The developed BPWHW/AgNW specifically captured paraquat (PQ) compared with other bipyridylium pesticides such as diquat (DQ) and difenzoquat (DIF). Further, field application test was carried out using hand-held Raman spectrometer on PQ pre-treated apple peels demonstrating the feasibility of paper-based SERS substrate for on-site residual pesticide detection. The developed M13 bacteriophage-functionalized AgNWs SERS sensor is expected to be applied for various pesticides and chemicals detection through modification of the M13 bacteriophage surface peptide sequences.