BS3.4 - H2O2 and Glutamate Imaging with Improved Sensitivity Based on Charge-Transfer-Type Potentiometric Redox Sensor Arrays
- Event
- 17th International Meeting on Chemical Sensors - IMCS 2018
2018-07-15 - 2018-07-19
Vienna, Austria - Chapter
- Biosensors 3
- Author(s)
- Y. Okumura, T. Iwata, K. Okumura, H. Shiguma, T. Horio, H. Doi, K. Takahashi, K. Sawada - Toyohashi University of Technology, Toyohashi (Japan)
- Pages
- 141 - 142
- DOI
- 10.5162/IMCS2018/BS3.4
- ISBN
- 978-3-9816876-9-9
- Price
- free
Abstract
Toward imaging the extrasynaptic distribution of glutamate (Glu) in brains, label-free potentiometric redox sensor arrays with 128 × 128 pixels have been developed. The Glu is detected through H2O2 production by an enzyme reaction and the oxidation of a mediator by H2O2. In this study, an adhesion layer of titanium was employed for the deposition of a gold layer, which is used to detect redox potential. The sensitivity to redox potential greatly increased, reaching near the theoretical value compared with the previous device. Due to a small variation in the sensitivity among the pixels, the distributions of H2O2 and Glu were successfully visualized using a poly-ion complex membrane entrapping enzymes and a mediator of ferrocenyl methanol. Furthermore, the limit of detection was achieved to be 10−6 M for both H2O2 and Glu.