ES2.3 - Precipitate-Assisted Signal Amplification of Surface Acoustic Wave Immunosensor for Mite Allergen

17th International Meeting on Chemical Sensors - IMCS 2018
2018-07-15 - 2018-07-19
Vienna, Austria
Emerging Systems 2 - Sensing Strategies
K. Toma, K. Oishi, T. Arakawa, K. Mitsubayashi - Institute of Biomaterials and Bioengineering, Tokyo Medical and Dental University, Tokyo (Japan), K. Kodaira, N. Kanamori - Bunkyo Gakuin University, Tokyo (Japan), N. Yoshimura, H. Yatsuda - Japan Radio Co. Ltd., Fujimino, Saitama (Japan)
316 - 317


House dust mites (HDM) cause allergic diseases such as allergic asthma and rhinitis. In order to reduce the risk of sensitization, an airborne HDM monitoring system that is composed of a sampler and a sensor is expected to be useful. However, to meet the standard set by the World Health Organization, a high sensitivity of the sensor is required. Here, we propose a method to amplify the signal of surface acoustic wave (SAW) immunosensor for HDM allergen (Der f 2) based on a horse radish peroxidase (HRP)-mediated enzymatic reaction. 3,3-diaminobenzidine (DAB) used as a substrate was polymerized through this enzymatic reaction, and a precipitate was formed. The resultant precipitate increased a total mass in close vicinity of a sensing area surface of the SAW immunosensor, thereby a signal was able to be amplified. In measurement of Der f 2 using sandwich assay with HRP-conjugated detection antibody (HRP-dAb), 3.7-fold signal amplification compared to the output signal from HRP-dAb was attained. This proposed strategy holds a potential of further signal amplification, and is expected to be useful for airborne HDM allergen monitoring.