C8.4 - Disposable Biosensor Arrays with Integrated Microfluidics for Biomedical- and Biotechnology Process-Monitoring
- SENSOR+TEST Conferences 2011
2011-06-07 - 2011-06-09
- Proceedings SENSOR 2011
- C8 - Bio-Chemo Sensors
- G. Jobst, I. Moser - Jobst Technologies GmbH, Freiburg (Germany)
- 516 - 519
Metabolic research both in vivo and in vitro, emerging therapeutic praxis of metabolic typing of cells and tissues, bioprocess development and fermentation control would benefit from a timely knowledge of metabolic parameters, e.g. glucose, glutamine, lactate, etc. - enabling adequate intervention, maximisation of product yield, and providing tools for the reliable and traceable processing of cells used as therapeutics.
A generic technology platform based on polymer laminate technologies with integrated electrochemical biosensors utilizing nano-composite membranes allows the fabrication of fully flexible devices comprising biosensor arrays. The flexible manufacturing technologies used for these devices also allow a cost effective customisation of them. Performance and applications of devices fabricated by this generic platform technology will be presented.
Devices for the simultaneous monitoring of lactate, pyruvate, glucose, glutamine, and the flow rate were realized with the biosensor array integrated in a flow-through cell with a total internal volume of 0.15 μl and less. The devices also provide ultra-low volume fluidic connection extensions enabling a highly variable use of it.
Performance of these devices shows physiological dynamic ranges, good to acceptable precision and operational stabilities of minimally two weeks for continuous operation for all the biosensors in the array. Flow rates used range from 0.1 to 100 μl/min. These systems were successfully applied for monitoring of cell cultures.