P2.1.2 Performance Study of a Potentiometric Sensor Array for Lactic Proteins Analysis
- Event
- 14th International Meeting on Chemical Sensors - IMCS 2012
2012-05-20 - 2012-05-23
Nürnberg/Nuremberg, Germany - Chapter
- P2.1 Biosensors
- Author(s)
- L. Dias - CIMO - Escola Superior Agrária, Instituto Politécnico de Bragança (Portugal), A. Veloso - DEQB – Instituto Superior de Engenharia de Coimbra (Portugal) and IBB – Centre of Biological Engineering, University of Minho, Braga (Portugal);, A. Machado - LAQUIPAI - Departamento Química, Faculdade de Ciências, Universidade do Porto (Portugal), A. Peres - LSRE - Laboratory of Separation and Reaction Engineering - Associate Laboratory LSRE/LCM, Escola Superior Agrária, Instituto Politécnico de Bragança (Portugal)
- Pages
- 1332 - 1335
- DOI
- 10.5162/IMCS2012/P2.1.2
- ISBN
- 978-3-9813484-2-2
- Price
- free
Abstract
Lactic proteins are complex biomolecules, possessing different molecular weights (from 19 kDa up to 66 kDa), which can be used as biomarkers for detecting food adulterations. This study reports for the first time the evaluation of two potentiometric sensor arrays, one electronic tongue (ET) using only cross-sensitivity chemical sensors and a hybrid-ET containing ionic-selective and cross-sensitivity chemical sensors. Although both devices could detect and distinguish soluble and insoluble lactic proteins, namely bovine serum albumin and three main casein fractions (alpha-, beta- and kappa-), the inclusion of ionic-selective sensors increased the device performance (100% of correct leave-oneout cross-validation classification for hybrid-ET against 94% for the ET) and reduced the number of sensors needed (5 for the hybrid-ET instead of 9 sensors used by the ET device). Finally, the results showed that with the hybrid-ET a second-order polynomial equation could be derived relating the measured potential of the aqueous solution and the logarithmic of the total casein concentration, with satisfactory determination coefficients (R2>0.99).