P5.3 - Microfluidic Measurement of Cell Motility in Response to Applied Non-homogeneous DC Electric Fields
- 12. Dresdner Sensor-Symposium 2015
2015-12-07 - 2015-12-09
Hotel Elbflorenz, Dresden
- P5. Mikrofluidische und nanostrukturierte Sensoren
- M. Rio, S. Bola, R. Funk, G. Gerlach - TU Dresden/D
- 191 - 195
Endogenous electric fields play an important role in many biological processes. In order to gain an insight into these biological phenomena, externally applied electric fields are used to study cellular responses. In the present study, we report on the steps towards microfluidic biochip assembling and its application to murine photoreceptor-derived 661W cells. In this work we describe the construction of a microfluidic biochip, which intends to mimic the non-homogeneous endogenic electric fields near wounds in vitro. The device is easy to fabricate and enables live-cell imaging under an inverted microscope. The experimental results have shown that the microfluidic biochip is biocompatible and suitable for cellular electrotaxis experiments in non-homogeneous DC electric fields.