BS5.1 - Biosensor of stress hormone cortisol based on molecular imprinted polymer
- Event
- 17th International Meeting on Chemical Sensors - IMCS 2018
2018-07-15 - 2018-07-19
Vienna, Austria - Chapter
- Biosensors 5 - Biomimetic Approaches
- Author(s)
- S. Klangphukhiew, R. Patramanon - Department of Biochemistry, Faculty of Science, Khon Kaen University, Khon Kaen (Thailand), R. Srichana - Molecular Recognition Materials Research Unit, Department of Pharmaceutical Chemistry, Faculty of Pharmaceutical Sciences, Prince of Songkla University, Hat Yai (Thailand), P. Lieberzeit - Department of Physical Chemistry, Faculty of Chemistry, University of Vienna, Vienna (Austria)
- Pages
- 207 - 208
- DOI
- 10.5162/IMCS2018/BS5.1
- ISBN
- 978-3-9816876-9-9
- Price
- free
Abstract
Cortisol is one of the key hormones that is involved in stress response and an indicator of chronic psychological disorders. It is produced by zona fasciculata and can be found in blood serum and saliva. This study describes the fabrication of cortisol biosensor using molecular imprinted polymers (MIPs) as recognition unit. The 642.3±35.8 nm-core shell cortisol-MIP was prepared by methacrylic acid and acrylamide with cortisol as a target molecule in a ratio of 2:1:1. Electron and atomic force microscope techniques were used to characterize the physical properties of cortisol-MIP. The binding characteristics were evaluated using covalent patterns changing in Fourier transform infrared (FTIR) spectra and massive response by quartz crystal microbalance (QCM) technique. Cortisol-MIP was integrated into the sensor and coated on the disposable screen-printed carbon electrode (SPCE) for portable electrochemical analysis, which displayed 1.28-20mM detection range and high selectivity compared to three other steroid analogs.