BS5.3 - Piezoelectric quartz crystal aptamer biosensor for detection and quantification of SARS CoV helicase protein
- 17th International Meeting on Chemical Sensors - IMCS 2018
2018-07-15 - 2018-07-19
- Biosensors 5 - Biomimetic Approaches
- D. Albano - Research Center for the Natural and Applied Sciences, University of Santo Tomas, Manila (Philippines), K. Shum, J. Tanner - Department of Biochemistry, The University of Hong Kong, Hong Kong (China), Y. Fung - Department of Chemistry, The University of Hong Kong, Hong Kong (China)
- 211 - 213
To meet the highly demanding challenge for detecting protein biomarkers at pg/mL level in highprotein sera sample, the coupling of paramagnetic nanoparticle technology with aptamer-PQC biosensor was attempted for developing aptamer-PQC biosensor for selective detection of SCV helicase protein produced from SARS CoV replication. The coupling of aptamer coated paramagnetic nanoparticles for sample pretreatment to aptamer-PQC biosensor has shown to detect helicase protein in one-minute assay with a detection limit of 3.5 ng/mL. The aptamer-coated crystal exhibits a frequency shift linearly proportional to the concentration of SARS helicase from 0.05 to 1 μg/mL with a correlation coefficient of 0.9975 and a repeatability of 6.8% (%RSD, n=3). After the enrichment procedure, recoveries of 102% and 119% were achieved using samples spiked with SARS helicase at concentrations of 10 ng/mL and 1.0 ng/mL respectively. It is the first report to detect for SCV helicase protein using PQC biosensor at pg/mL level after magnetic bead enrichment.