BS6.3 - A label-free assay for sensitive detection of trypsin by using gold nanoclusters as fluorescent probes
- Event
- 17th International Meeting on Chemical Sensors - IMCS 2018
2018-07-15 - 2018-07-19
Vienna, Austria - Chapter
- Biosensors 6 - Nanoparticles
- Author(s)
- X. Yan, H. Li, G. Lu - State Key Laboratory on Integrated Optoelectronics, College of Electron Science and Engineering, Jilin University, Changchun (China)
- Pages
- 308 - 309
- DOI
- 10.5162/IMCS2018/BS6.3
- ISBN
- 978-3-9816876-9-9
- Price
- free
Abstract
Herein, we designed a convenient and label-free fluorescence nanosensor for sensitive detection of trypsin (TRY). The fluorescence of gold nanoclusters (AuNCs) could be quenched by cytochrome c (Cyt c) via electron transfer (ET) mechanism. However, TRY specifically catalyzes the hydrolysis of Cyt c to small peptide fragments, causing sufficient recovery of fluorescent signal. The proposed system displayed a sensitive and selective response to TRY in the range from 0.2 to 100 µg mL-1, with a detection limit of 0.08 µg mL-1. The designed platform has been successfully applied to the accurately detection of TRY activity in human serum samples and urine samples with satisfactory results. Significantly, the sensing probe has been used to construct paper-based test strips for rapid and visual detection of TRY with highly promising, which validates the potential point-of-care application.