BS6.4 - A fluorescent biosensor based on molybdenum disulfide (MoS2) nanosheets and protein aptamer for sensitive detection of carcinoembryonic antigen

17th International Meeting on Chemical Sensors - IMCS 2018
2018-07-15 - 2018-07-19
Vienna, Austria
Biosensors 6 - Nanoparticles
L. Zhao, M. Cheng, G. Liu, H. Lu, Y. Gao, G. Lu - State Key Laboratory on Integrated Optoelectronics, College of Electronic Scienand Engineering, Jilin University, Changchun (China)
310 - 311


Simple, rapid, sensitive detection of tumor biomarker carcinoembryonic antigen (CEA) is of great importance for the screening, diagnosis and prognosis evaluation of various gastroenteric tumor. This paper presents a "turn-on" flourescent biosensor based on molybdenum disulfide(MoS2) nanosheets and fluorophore labeled protein aptamer for rapid and sensitive detection of CEA protein. CEA aptamer probe can be adsorbed on the surface of MoS2 nanosheets in close proximity via van der Waals force, triggering fluorescence resonance energy transfer, and consequently fluorescence signal of aptamer probe was quenched. While in the presence of CEA protein, the fluorescence signal was recovered because aptamer probe could detach from MoS2 nanosheets with binding-induce conformation change. The concentrations of target CEA protein can be determined by monitoring the turn on signal of fluorescence. The sensing platform was utilized to detect CEA with a broad range of 100 pg/mL-100 ng/mL with the detection limit of 34 pg/mL, and simultaneously exhibited good stability, reproducibility and high selectivity. The aptamer-MoS2 based fluorescent biosensor may be an ideal mode for protein detection in clinical sample, pesticide detection and environmental monitoring.