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T5.3.2 - Fiber optic sensors based on Mach-Zehnder Interferometer for the detection of bacterial biofilms

Event
EUROSENSORS XXXVII
2025-09-07 - 2025-09-10
Wroclaw
Band
Lectures
Chapter
T5.3 - Biochemicaland Environmental Sensors 1
Author(s)
A. Rashidi, F. Esposito, A. Srivastava, S. Campopiano, A. Iadicicco - University of Naples "Parthenope", Naples (Italy), A. Sacco, G. Di Prisco - Institute for Composites and Biomaterials-CNR, Portici (Italy) Sustainable Plant Protection-CNR, Portici (Italy), F. Granata, G. Coppola - Institute of Applied Science and Intelligent Systems-CNR, Naples (Italy), C. Vitagliano, M. Giordano, L. Sansone - Institute for Polymers Composites, and Biomaterials-CNR, Portici (Italy)
Pages
138 - 139
DOI
10.5162/EUROSENSORS2025/T5.3.2
ISBN
978-3-910600-07-2
Price
free

Abstract

In this work, a chemical sensor based on an In-fiber optic Mach-Zehnder Interferometer (MZI) is re-ported. The MZI is fabricated using standard single-mode fiber with a waist-enlarged splicing method, where the spliced point acts as a coupling and re-coupling element for cladding modes and core mode. Unlike conventional transmission based MZIs, a reflective configuration is prepared by coating the fiber tip with silver developing a convenient fiber optic probe. A sensitivity of about 125 nm/RIU to the sur-rounding medium refractive index is obtained. As an application, the biofilm growth of a Gram-negative aerobic bacterium, i.e. Pseudomonas alcaligenes, is observed by monitoring the resonance wavelength shifts in real-time over three days. Biofilm thickness is further analyzed by using Atomic Force Micros-copy technique demonstrating the effectiveness of fiber optic technology for real time continuous moni-toring of biofilm growth.

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