D2-3.3 - Label-Free Electrochemical Biosensor for Real-Time Monitoring of Erythropoiesis via Glycophorin A (CD235a) Detection

Event

23. ITG/GMA-Fachtagung Sensoren und Messsysteme 2026
2026-06-09 - 2026-06-10
Nürnberg

Band

Vorträge

Chapter

Biosensoren

Author(s)

S. M. Hesari, L. Deckert, U. Steinmann - Otto-von-Guericke-Universität Magdeburg, Magdeburg, C. von Heckel, J. N. B. Rios - Otto-von-Guericke-Universität Magdeburg, Magdeburg

Pages

226 - 232

DOI

10.5162/sensoren2026/D2-3.3

ISBN

978-3-910600-11-9

Price

free

Abstract

Electrochemical biosensors offer an alternative method for identification of specific proteins in a biological sample by producing an electrical signal that is dependent on molecular recognition. Herein, we present a label-free electrochemical biosensor that focuses on Glycophorin A (CD₂₃₅a). The biosensor is based on gold microelectrodes which were functionalized with a self-assembled monolayer (SAM) of mercaptoundecanoic acid (MUA), then activated with EDC/NHS. Non-specific binding on the oriented immobilized anti-CD₂₃₅a antibodies was blocked using bovine serum albumin (BSA). These sequential surface modifications yielded a very low characteristic current by cyclic voltammetry (CV): The peak current significantly decreased by 93.4% (anodic) and 96.9% (cathodic) between the bare electrode and BSA-blocked surface. Analysis of the impedance spectra revealed a charge transfer resistance (Rct) of 6 kΩ for bare electrode. This increased significantly to 434 kΩ following the detection of CD₂₃₅a, indicating successful target binding. This substantial increase in Rct confirms the biosensor's high sensitivity. Furthermore, the biosensor demonstrated high specificity towards the CD₂₃₅a surface antigen.