D2-3.4 - Modular, 3D-Printed Organ-on-Chip Platform with Integrated Optical Sensing for Real-Time Monitoring of the Tumor Microenvironment
- Event
- 23. ITG/GMA-Fachtagung Sensoren und Messsysteme 2026
2026-06-09 - 2026-06-10
Nürnberg - Band
- Vorträge
- Chapter
- Biosensoren
- Author(s)
- M. Wilfred, L. Deckert, U. Steinmann - Otto-von-Guericke-Universität Magdeburg, Magdeburg
- Pages
- 233 - 239
- DOI
- 10.5162/sensoren2026/D2-3.4
- ISBN
- 978-3-910600-11-9
- Price
- free
Abstract
Conventional two-dimensional (2D) cancer culture systems fail to reproduce key aspects of the tumor microenvironment (TME), including oxygen gradients, biomechanical cues, and three-dimensional (3D) architecture. Although 3D spheroids and organoids provide improved physiological relevance, their static culture conditions limit dynamic perfusion and hinder real-time environmental monitoring. Existing microfluidic Organ-on-chip (OoC) platforms address these limitations but remain inaccessible to many laboratories due to high fabrication costs, reliance on cleanroom facilities, and lack of intermediate access to cultures. To overcome these challenges, we present a modular, reusable OoC platform fabricated using stereolithography (SLA) 3D printing. This approach enables rapid, cost-effective production of a perfusion system without specialised infrastructure. The system incorporates plug-and-play elements, including a screw top, polydimethylsiloxane (PDMS) membrane support, and central perfusion chamber, allowing easy assembly, sterilisation, and direct access to cultures during experiments. An integrated optical oxygen sensor enables continuous, non-invasive monitoring of oxygen partial pressure (pO ), providing quantitative insight into metabolic activity and hypoxia formation under perfusion. This platform supports long-term perfusion culture of 3D cancer organoids, enhances environmental monitoring, and improves accessibility of advanced in vitro models. It represents a significant step toward scalable, instrumented systems for cancer research and drug screening. ₂