3.2.1 Designing an interface and cell for cellular biosensing
- 14th International Meeting on Chemical Sensors - IMCS 2012
2012-05-20 - 2012-05-23
- 3.2 Biosensors III (cell based)
- T. Harayuma - Department Biological Functions and Engineering, Kyushu Institute of Technology, Kitakyushu Research and Science Park (Japan)
- 262 - 264
Cells are very intelligent and smart transducers because they transmit various molecular signals in response to extracellular stimuli, both chemically and physically. This fact suggests that living cells can act as sensors in qualified analysis. Such cellular biosensors for qualified analyses can be employed as smart tool in high-throughput analysis in clinical drug discovery. Clinical drug discovery in most cases begins with molecular screening in order to select a lead substance. This process is a key step in successful drug development. Lead substances are identified on the basis of their ability to affect objective biological properties. In order to judge the molecular efficacy of a lead substance, both animal experiments and cell-based bioassays have been employed. In high-throughput assays, cellular biosensing is one of the “smart” methods. However, it is not easy to perform sensing of cellular signals in cell culture conditions. Here, we present some tactics to perform practical cellular biosensing through designing both a molecular interface on the sensor and the cell itself.