3.2.4 [pH]o imaging in proton releasing cells by an ion image sensor-based chemical microscopy
- 14th International Meeting on Chemical Sensors - IMCS 2012
2012-05-20 - 2012-05-23
- 3.2 Biosensors III (cell based)
- T. Sakurai - Electronics-Inspired Interdisciplinary Research Institute, Toyohashi University of Technology (Japan), H. Taki, K. Okumura, K. Sawada - Department of Electrical and Electronic Information Engineering, Toyohashi University of Technology; Susumu Terakawa, JST CREST, Tokyo (Japan), Y. Fukushi - Medical Photonics Researce Center, Hamamatsu University, School of Medicine (Japan)
- 273 - 274
To examine the functions of proton release from the cells, we measured the extracellular pH ([pH]o) by using an ion image sensor made of a 2 dimensional array of potential sensitive pixels. We recorded the changes in [pH] o of cells such as osteoclasts, gastric glands and hippocampal slices. After stimulation of cells and/or tissues, [pH]o was decreased, and the degree of the pH changes was dependent on the doses of stimulants. We established a label free microscopic assessment of 2Ddistributions of biologically interesting substances by the present chemical sensing technique. The [pH] o imaging by chemical microscope promises the usefulness in the medical fields for the analysis of diseases such as osteoporosis, gastric ulcer, and stroke.