2025 SMSI Bannerklein

5.1.1 Detection of pathogenic Staphylococcus aureus bacteria by Electrochemical Impedance

Event
14th International Meeting on Chemical Sensors - IMCS 2012
2012-05-20 - 2012-05-23
Nürnberg/Nuremberg, Germany
Chapter
5.1 Impedance-based Sensing
Author(s)
M. Braiek, A. Maaref - Laboratoire de Physique et Chimie des Interfaces, Faculté des Sciences de Monastir (Tunisia), N. Jaffrezic-Renault - Laboratoire des Sciences Analytiques, UMR CNRS 5180, Université Claude Bernard-Lyon1 (France), K. Bekir Rokbani, B. Mrabet, A. Bakhrouf - Laboratoire d’Analyse, Traitement et Valorisation des Polluants de l’Environnement et des Produits, Faculté de Pharmacie de Monastir (Tunisia)
Pages
418 - 420
DOI
10.5162/IMCS2012/5.1.1
ISBN
978-3-9813484-2-2
Price
free

Abstract

The timely detection of pathogens is a subject of great importance. In this work, the objective is to elaborate an immunosensing system for detection and quantification of Staphylococcus aureus. Poyclonal anti-S. aureus are immobilized onto gold electrodes via chemical bond formation between antibody amino groups and a carboxylic acid containing self-assembled molecular monolayer. The evaluation of the developed immunosensor performance was accomplished through the monitoring of the electron-transfer resistance detected by electrochemical impedance spectroscopy in the presence of [Fe(CN)63-]/[Fe(CN)6 4- ] as redox probe. A low detection limit of 10 fcu/ml and a linear range up to 10^7 cfu/ml were obtained.

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