2025 SMSI Bannerklein

5.1.4 Impedance-Based Immobilized Enzyme Biosensor for Detection of Organophosphates

Event
14th International Meeting on Chemical Sensors - IMCS 2012
2012-05-20 - 2012-05-23
Nürnberg/Nuremberg, Germany
Chapter
5.1 Impedance-based Sensing
Author(s)
M. Smiechowski, A. Ward - Guild Associates, Inc. (USA)
Pages
424 - 427
DOI
10.5162/IMCS2012/5.1.4
ISBN
978-3-9813484-2-2
Price
free

Abstract

This paper describes the development of an electrochemical impedance-based biosensor for the detection of organophosphates. The biosensor consisted of a carbon paste electrode, modified with an immobilized organophosphate-sensitive enzyme layer. The enzyme was immobilized through crosslinking with glutaraldehyde, polyethyleneimine, and additional cell material. The sensor signal and response to paraoxon substrate was tested versus two different electrode arrangements and over several applied dc potentials. The sensor was capable of measuring 10 µM paraoxon in buffer solution. Stability of the sensor stored in air over time was investigated. Results are shown for the biosensor versus a paraoxon substrate operating in 50 mM HEPES buffer at 150 mV, 0 mV, and –150 mV vs. Ag/AgCl.

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