8.1.2 Recording of living cell membrane depolarisation with AlGaN/GaN sensor

Event
14th International Meeting on Chemical Sensors - IMCS 2012
2012-05-20 - 2012-05-23
Nürnberg/Nuremberg, Germany
Chapter
8.1 Chemical Sensors based on III-V Semiconductors
Author(s)
A. Podolska, G. Parish, B. Nener - School of Electrical, Electronic and Computer Engineering, The University of Western Australia (Australia), R. Seeber, K. Pfleger - Laboratory for Molecular Endocrinology - GPCRs, Western Australian Institute for Medical Research (WAIMR), and Centre for Medical Research, The University of Western Australia (Australia), U. Mishra - Department of Electrical and Computer Engineering, University of California (USA)
Pages
665 - 666
DOI
10.5162/IMCS2012/8.1.2
ISBN
978-3-9813484-2-2
Price
free

Abstract

This work reports on AlGaN/GaN-based sensor development and application to biological sensing. Living cells were seeded on the sensitive region of encapsulated devices for 30 minutes before membrane depolarisation with KCl. To confirm cell activity KCl was also added to devices in physiological salt solution with no cells. The resulting responses were recorded in both cases and demonstrated significant differences in profile and amplitude. Since the AlGaN/GaN structure itself is ion-sensitive, a chemical signal is expected even in the absence of cells. The depolarisation signal observed for the devices with cells is thus expected to incorporate this chemical response.

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