P1.2.3 Development of a Love-wave Immunosensor with Microfluidic Technology to Detect Phages in Dynamic Mode
- Event
- 14th International Meeting on Chemical Sensors - IMCS 2012
2012-05-20 - 2012-05-23
Nürnberg/Nuremberg, Germany - Chapter
- P1.2 Resonant Sensors
- Author(s)
- D. Matatagui, M. Fernández, J. Fontecha, J. Santos, M. Horrillo - GRIDSEN, CSIC (Spain), D. Moynet - Université de Bordeaux, Laboratoire d'immunologie (France), J. Esquivel, I. Gràcia, C. Cané - Instituto de Microelectrónica de Barcelona, CSIC (Spain), C. Déjous, D. Rebière - Université de Bordeaux, IMS, IPB-ENSEIRB (France)
- Pages
- 892 - 895
- DOI
- 10.5162/IMCS2012/P1.2.3
- ISBN
- 978-3-9813484-2-2
- Price
- free
Abstract
The goal has been to develop an immunosensor based on a Love-wave device and a PDMS microfluidic chip in order to detect potentially pathogenic microorganisms in real time. Therefore, it has been tested with the phage M13 detected through the mouse monoclonal antibody anti-M13 (AM13). The Double-layer Agar (DLA) technique has been used to quantify the number of phages, linked to the sensor surface, in plaques-forming unit (pfu); that has allowed us to compare the number of phages determined by DLA and the estimated by the frequency shift, and both were very similar. With the aim to obtain a short response time, the detection of the phage was carried out in dynamic mode. The range tested concentrations has varied between 2x10^10 pfu·ml^-1 and 5x10^8 pfu·ml^-1. A fast response and a good discrimination among concentrations have been achieved. Besides, the AM13 has been used as secondary antibody in order to measure lower concentrations, such as 5·10^7 pfu·ml^-1.