2.2.5 Microarrayed 2D-SPR Immunosensor for Interleukin-2
- Event
- 14th International Meeting on Chemical Sensors - IMCS 2012
2012-05-20 - 2012-05-23
Nürnberg/Nuremberg, Germany - Chapter
- 2.2 Biosensors II (DNA, SPR)
- Author(s)
- M. Suzuki, Y. Kawauchi, Y. Iribe - Faculty of Engineering, University of Toyama (Japan)
- Pages
- 178 - 180
- DOI
- 10.5162/IMCS2012/2.2.5
- ISBN
- 978-3-9813484-2-2
- Price
- free
Abstract
This paper reports high sensitive detection of mouse interleukin-2 (IL-2) in 0.010 mm i.d. wells, single cell based microwell array, by using a high resolution two dimensional surface plasmon resonance (2D-SPR) sensor and enzyme catalyzed biochemical amplification of SPR signal. In order to detect cytokines from a single cell, sensitivity of < ng/ml should be required even if we consider the small volume of microwells (~1pl). In this study, alkaline phosphatase (ALP) catalyzed precipitation reaction was employed and 0.2 ng/ml IL-2 could be successfully detected both in buffer solution and culture medium.