DH1.3 - Validation of Sterilization Methods by Means of Gas Sensors and Spore-based Biosensors
- 17th International Meeting on Chemical Sensors - IMCS 2018
2018-07-15 - 2018-07-19
- Sensors for Diagnostics and Health Care 1
- J. Arreola, J. Oberländer, F. Vahidpour, M. Schöning - Institute of Nano- and Biotechnologies (INB), Aachen University of Applied Sciences, Jülich (Germany), M. Keusgen - Institute of Pharmaceutical Chemistry, Marburg (Germany)
- 115 - 116
In aseptic food industry, hydrogen peroxide is widely used as sterilization agent. The state-of-the-art methods used for the validation of sterilization procedures are microbiological tests, where highly resistant spores are exposed to the sterilant under investigation. The logarithmic kill rate of the spores is determined and the sterilization can be subsequently assured. However, this is tedious and slow; the results can be obtained at the earliest after 24 hours (usually even after 72 hours, see e.g., count reduction test). For this reason, rapid and efficient sterilization methods are needed. In this work, we present two different sensors to validate sterilization procedures: a calorimetric gas sensor for monitoring hydrogen peroxide concentrations and a spore-based biosensor to evaluate the viability of the spores.