BS7.2 - 3D cardiomyocyte-based biosensor with tissue engineering scaffold and microelectrode array

17th International Meeting on Chemical Sensors - IMCS 2018
2018-07-15 - 2018-07-19
Vienna, Austria
Biosensors 7 - Cell Sensing
X. Wei, Q. Wu, Y. Pan, Z. Qin, N. Hu, P. Wang - Biosensor National Special Laboratory, Key Laboratory for Biomedical Engineering of Education Ministry, Department of Biomedical Engineering, Zhejiang University, Hangzhou (China), Q. Gao - State Key Laboratory of Fluid Power and Mechatronic Systems, Key Laboratory of 3D Printing Process and Equipment of Zhejiang Province, College of Mechanical Engineering, Zhejiang University, Hangzhou (China)
336 - 337


Polylactic acid (PLA) and polycaprolactone (PCL) were selected as materials to fabricate tissue engineering scaffolds by 3D printing and electrospinning, which were used to culture cardiomyocytes of neonatal rats. Then the scaffolds with cardiomyocytes were coupled with microelectrode array (MEA) to form a 3D cell-based biosensor, which was used to detect the extracellular field potential (EFP) of cardiomyocytes. The experimental results demonstrated that cardiomyocytes adhered and grew well in scaffolds, and could drive fibers to produce combined beating due to the excitationcontraction coupling. After 48 hours, the beating rate of cardiomyocytes in the scaffolds tended to be stable. The detecting results demonstrated that scaffolds and MEA were coupled well to be a 3D cellbased biosensor system, which could detect the EFP of cardiomyocytes in scaffolds with stable and high-SNR signals, and the EFP amplitude and firing rate are both similar to the signals recorded from traditional 2D culturing method.