BS7.3 - Receptor-specific Bitterness Detection Using Immortal Cell Line and Impedance Sensor
- 17th International Meeting on Chemical Sensors - IMCS 2018
2018-07-15 - 2018-07-19
- Biosensors 7 - Cell Sensing
- C. Qin, Y. Pan, Z. Qin, P. Wang - Biosensor National Special Laboratory, Key Laboratory for Biomedical Engineering of Ministry of Education, Department of Biomedical Engineering, Zhejiang University, Hangzhou (China), L. Huang, D. Zhao - College of Life Sciences, Zhejiang University, Hangzhou (China)
- 338 - 339
Bitterness detection draws great attention in pharmaceutical and food industry. Existing detection methods relying on redox reactions lack specificity and most cell-based biosensors stay merely on cellular level thus ignore the specific receptor being activated. In this study, an impedance sensor is built to detect bitterness receptor-specifically using Caco-2 cell line, which expresses human T2R38 endogenously. After parameter optimization, this sensing system generates dose-dependent responses to known T2R38 ligands, phenylthiocarbamide and propylthioutacil. Quantitative study shows that the limits of detection are 0.094μM and 0.84μM respectively. Afterwards, specificity is also checked by other taste qualities and bitter compounds which cannot activate T2R38. In conclusion, this receptor-specific biosensor provides new platform for not only bitterness detection but also ligands detection, which is important in study such as mechanism of bitter perception and bitter masking.