SM1.2 - Probing the Optical Pressure of Pathogen-Exposed Macrophages on a Microfluidic Chip
- 17th International Meeting on Chemical Sensors - IMCS 2018
2018-07-15 - 2018-07-19
- Sensor Systems and Microfluidics 1
- G. Collins, D. Barlow, B. Giordano, Q. Lu - Naval Research Laboratory, Washington (USA), D. Haridas - American Society for Engineering Education, Washington (USA)
- 366 - 367
Optical force can be utilized to achieve label-free sensing and separation by utilizing this force in conjunction with the drag forces from a fluid inside the channel of a microfluidic chip. The custom microfluidic chip discussed here is comprised of five layers, incorporating a 3D hydrodynamic focusing nozzle, an injection channel, and an exit channel which can be branched into two or more channels for cell sorting and collection. The exposure of macrophages, a type of innate immune cell which plays an important role in the body’s early line defense against biological invasion, to lipopolysaccharides (LPS) results in significant cell population shifts in terms of decreased average velocities when compared to control cells. Confocal microscopy, atomic force microscopy (AFM) and Raman spectroscopy are utilized as complementary tools to study changes in the macrophage cells upon exposure to LPS and help explain the velocity changes observed within the microfluidic chip.