SM1.4 - Lab-on-a-Chip System to Monitor the Oxygen Consumption of Mammalian Cells
- 17th International Meeting on Chemical Sensors - IMCS 2018
2018-07-15 - 2018-07-19
- Sensor Systems and Microfluidics 1
- F. Bunge, S. van den Driesche, M. Vellekoop - Institute for Microsensors, -actuators and -systems (IMSAS), MCB, University of Bremen (Germany), A. Waite - Alfred-Wegener-Institute (AWI), Bremerhaven (Germany), U. Mirastschijski - Centre for Biomolecular Interactions, University of Bremen (Germany)
- 370 - 371
We present a Lab-on-a-Chip system to monitor the uptake of oxygen by mammalian cells during their metabolism. The oxygen consumption indicates the state of the cells and their response to the physical or chemical stimuli and is thus of interest for biological analyses. Although oxygen measurements are topic of investigations for a long time already, such a measurement system is still complex with a high number of external components which hamper their usage for biological researchers. Our system consists of a microfluidic chip with an oxygen sensitive phosphorescent film out of dye PtTFPP in a matrix of polystyrene. All external components of the optical system and the temperature control are low-cost components that are integrated in the assembly. Therefore, the entire system has a small footprint, an easy usage and a high degree of automation. With this system, the oxygen uptake of HaCaT cells is reproducibly measured as 10, 20 and 22 amol/(cell∙s) for temperatures of 34°C, 37°C and 40°C.