W7.3.2 - A 3D printed RT-LAMP microdevice for the detection of feline calicivirus
- Event
- EUROSENSORS XXXVII
2025-09-07 - 2025-09-10
Wroclaw - Band
- Lectures
- Chapter
- W7.3 - Novel Technologies and Applications of Sensors 3
- Author(s)
- W. Kubicki, J. Kornicka, R. Walczak - Wroclaw University of Science and Technology, Wroclaw (Poland)
- Pages
- 183 - 184
- DOI
- 10.5162/EUROSENSORS2025/W7.3.2
- ISBN
- 978-3-910600-07-2
- Price
- free
Abstract
This study presents preliminary results of the development and characterization of a 3D-printed micro-fluidic lab-on-a-chip (LOC) device for rapid molecular diagnostics of feline calicivirus (FCV). The device was fabricated using digital light processing (DLP) technology and printed with biocompatible GR-10 photopolymer resin. The LOC was successfully applied to perform a reverse transcription loop-mediated isothermal amplification (RT-LAMP) assay for the detection of FCV, a common pathogen in cats. The results of the RNA amplification reactions confirmed the high potential of 3D printing technology and the applied UV-curable resin with isothermal enzymatic reactions for prospective use in veterinary medicine.