TP11 - Direct-printed amiodarone electrochemical sensor. Identi-fication of DNA based bioreceptor
- Event
- EUROSENSORS XXXVII
2025-09-07 - 2025-09-10
Wroclaw - Band
- Poster II
- Chapter
- Bio/chemical and Environmental Sensors
- Author(s)
- M. Urbanowicz, A. Paziewska-Nowak, K. Bobrowska, A. Sołdatowska, M. Dawgul, D. G. Pijanowska - Polish Academy of Sciences, Warsaw (Poland)
- Pages
- 371 - 372
- DOI
- 10.5162/EUROSENSORS2025/TP11
- ISBN
- 978-3-910600-07-2
- Price
- free
Abstract
This study focuses on the electrochemical behaviour of amiodarone, a widely used anti-arrhythmic drug, to develop a direct-printed electrochemical sensor. The oxidation of amiodarone was confirmed via voltammetric techniques. A comparative analysis of graphite electrodes fabricated using direct printing (DPE) and screen printing (SPE) technologies revealed the superior performance of DPE sensors, with a sensitivity of 1.403 µA/µM – nearly twice that of SPE-based sensors. In the second part of the study, we screened for double-stranded DNA sequences capable of forming complexes with amiodarone. High-performance liquid chromatography (HPLC) identified the 5’-AGCTATAAAT-3’ se-quence as a promising bioreceptor. The interaction between amiodarone and DNA was further con-firmed by NMR spectroscopy, based on diffusion coefficient measurements and chemical shift changes observed in NOESY and TOCSY spectra. These findings demonstrate the potential of integrating printed sensor technologies with molecular interaction studies for drug screening and biosensing applications.