TP64 - DNA-6MP interactions for electrochemical biosensor development
- Event
- EUROSENSORS XXXVII
2025-09-07 - 2025-09-10
Wroclaw - Band
- Poster II
- Chapter
- Sensors for Medicine and Life-Sciences
- Author(s)
- A. Sołdatowska, M. Urbanowicz, K. Sadowska, D. G. Pijanowska - Polish Academy of Sciences, Warsaw (Poland), M. Urbanowicz - National Medicines Institute, Warsaw (Poland)
- Pages
- 482 - 483
- DOI
- 10.5162/EUROSENSORS2025/TP64
- ISBN
- 978-3-910600-07-2
- Price
- free
Abstract
This study presents a novel analytical tool for the detection of 6-mercaptopurine (6MP) through the development of a DNA-based electrochemical biosensor. A specific double-stranded DNA sequence (5′-GGCAGGACGGAG-3′) was identified for its ability to selectively bind 6MP, with an affinity constant of 2.52 × 10³ M⁻¹, as confirmed by DOSY NMR. Selectivity was further verified by HPLC anal-ysis in the presence of common metabolites and co-administrated drugs. When immobilized on theelectrode surface, this DNA-based bioreceptor offers a rapid, cost-effective, and easy-to-use alternative to conventional analytical tools, with promising applications in therapeutic drug monitoring and person-alized medicine.