Mo-MLO-04 - Centrifugal Microfluidic Automation of Protein Sample Preparation for Nanopore Sensing

Event

EUROSENSORS 2026
2026-09-06 - 2026-09-09
Zurich

Band

Poster

Chapter

Microfluidic Devices, Lab on a Chip, µTAS, Organ on Chip Systems

Author(s)

C. Kleber, C. Hensel - University of Freiburg,Freiburg (Germany), I. Suppanz, G. Mittler - Max Planck Institute of Immunobiology and Epigenetics,Freiburg (Germany), M. Hinrichs, S. Gutsch - Hahn-Schickard,Freiburg (Germany), T. Hutzenlaub, F. von Stetten, N. Paust, A. Schreiber, J. -N. Klatt - University of Freiburg,Freiburg (Germany) und Hahn-Schickard,Freiburg (Germany)

Pages

493 - 494

DOI

10.5162/eurosensors2026/Mo-MLO-04

ISBN

978-3-910600-12-6

Price

free

Abstract

We present a microfluidic LabDisk automating the solid-phase-enhanced sample preparation (SP3) workflow for nanopore sensing. Using myelin basic protein (MBP), the system produces nanopore-com-patible samples preserving membrane stability and enabling reliable detection with the aerolysin AeL-R220S nanopore variant. A distinct MBP peptide marker supports confident identification. Automated and manual workflows show identical nanopore signals (I/I₀ ≈ 0.75, <0.2 % deviation) and comparable missed cleavage rates, demonstrating robust, standardized, and user-friendly sample preparation.