Tu-MLO-05 - A Microreactor Workflow for Spatial Proteomics Sample Preparation

Event

EUROSENSORS 2026
2026-09-06 - 2026-09-09
Zurich

Band

Poster

Chapter

Microfluidic Devices, Lab on a Chip, µTAS, Organ on Chip Systems

Author(s)

M. Metzger, T. Hutzenlaub, N. Paust, J. -N. Klatt - Hahn-Schickard,Freiburg (Germany), M. Maldacker, O. Schilling - Medical Center – University of Freiburg,Freiburg (Germany)

Pages

507 - 508

DOI

10.5162/eurosensors2026/Tu-MLO-05

ISBN

978-3-910600-12-6

Price

free

Abstract

We present MR-SP 2 [1] (microreactor-based sample preparation workflow for spatial proteomics) that addresses key loss mechanisms in laser capture microdissection (LCM) coupled to liquid chromatography–tandem mass spectrometry (LC-MS/MS). MR-SP 2 integrates specimen capture, onepot processing, and pipetting-free transfer to LC-MS/MS via Evotip precolumns. Evaluated using formalin-fixed paraffin-embedded (FFPE) murine kidney tissue, MR-SP 2 reduced the adsorptive surface area by 64% compared to tube-based workflows, yielding more than a 3-fold increase in protein identifications.