Mo-SMD-06 - Electrochemical and Electrochemiluminiscence in vitro Detection Methods of a Human Cytomegalovirus in Oncology Patients

Event

EUROSENSORS 2026
2026-09-06 - 2026-09-09
Zurich

Band

Poster

Chapter

Sensing For Medical Diagnostics And Monitoring

Author(s)

L. Moranova, A. Fried, K. Itterheimova, M. Bartosik - Masaryk Memorial Cancer Institute,Brno (Czech Republic), M. Hendrych - St. Anne's Univ. Hospital,Brno (Czech Republic)

Pages

596 - 597

DOI

10.5162/eurosensors2026/Mo-SMD-06

ISBN

978-3-910600-12-6

Price

free

Abstract

We report two electrochemical biosensors for rapid detection of oncogenic mutations based on rolling circle amplification and amperometric detection. The platform enables highly selective identification of mutations in KRAS and PIK₃CA , with sensitivity down to ~61 pM and <1% mutant allele fraction. An ultrafast hyperbranched RCA strategy reduces amplification time to 5 minutes while preserving singlenucleotide specificity. The method shows strong agreement with NGS and offers a rapid, cost-effective solution for point-of-care diagnostics.