P2EC.9 - Paper-Based Amperometric Sensor for Acetylcholinesterase Determination Using Screen-Printed Graphene Electrode
- Event
- 17th International Meeting on Chemical Sensors - IMCS 2018
2018-07-15 - 2018-07-19
Vienna, Austria - Chapter
- P-2 - Electrochemical Sensors
- Author(s)
- P. Panraksa, O. Chailapakul - Chulalongkorn University, Bangkok (Thailand), W. Siangproh - Srinakharinwirot University, Bangkok (Thailand), T. Khampieng, A. Apilux - Mahidol University, Nakhon Pathom (Thailand)
- Pages
- 719 - 719
- DOI
- 10.5162/IMCS2018/P2EC.9
- ISBN
- 978-3-9816876-9-9
- Price
- free
Abstract
Acetylcholinesterase (AChE) is an enzyme in cholinergic synapse which is an indicator of pesticides toxicity. Therefore, AChE activity determination is essential. In this work, a simple, rapid, sensitive, and selective paper-based electrochemical sensor for determination of AChE was firstly developed. A screen-printed graphene electrode (SPGE) was used as a working electrode due to its excellent electrical conductivity, electron mobility, high sensitivity, and cost effectiveness. The amperometric determination of AChE is based on the electrochemical signal of thiocholine (TCh) which is generated from the hydrolysis of acetylthiocholine (ATCh) by AChE. For AChE detection, ATCh immobilized sheet was stacked onto the detection sheet using double adhesive tape...