P1DH.4 - Miniaturized System with a Facile Microfluidic Chip for DNA Extraction, Isothermal Amplification and Fluorescence Detection
- Event
- 17th International Meeting on Chemical Sensors - IMCS 2018
2018-07-15 - 2018-07-19
Vienna, Austria - Chapter
- P-1 - Sensors for Diagnostics and Healthcare
- Author(s)
- X. Chen, L. Zhang, D. Cui - State Key Laboratory of Transducer Technology, Institute of Electronics, Chinese Academy of Sciences, Beijing (China)
- Pages
- 663 - 664
- DOI
- 10.5162/IMCS2018/P1DH.4
- ISBN
- 978-3-9816876-9-9
- Price
- free
Abstract
In this paper, a biomedical miniaturized system with a disposable microfluidic chip has been developed for solid phase extraction (SPE), loop-mediated isothermal amplification (LAMP) and realtime fluorescence detection. DNA was extracted from real biological samples, such as whole blood, by using a Glass-polydimethylsiloxane (PDMS)-glass microfluidic chip with a cell, which were also designed and fabricated for loop-mediated isothermal amplification (LAMP) in the same cell. LAMP reaction and real-time fluorescence detection were implemented by using the miniaturized system including a mini heating plate, a temperature sensor, a temperature controller, fluorescence detector and a adjustable mechanical stage. DNA has successfully extracted from whole blood and then amplification and detection. This biomedical system has the potential for point-of-care diagnostics (POCT) with the many advantages, such as not high cost, short analysis and detection time, not much reagent and sample consumption and so on.