P1DH.8 - An Exploration of Nucleic Acid Liquid Biopsy Using Glucose Meter

17th International Meeting on Chemical Sensors - IMCS 2018
2018-07-15 - 2018-07-19
Vienna, Austria
P-1 - Sensors for Diagnostics and Healthcare
J. Xu, W. Zhao, Y. Gu, C. Xu, H. Chen - State Key Laboratory of Analytical Chemistry for Life Science and Collaborative Innovation Center of Chemistry for Life Sciences, School of Chemistry and Chemical Engineering, Nanjing University, Nanjing (China)
670 - 671


The development of non-invasive techniques for the diagnosis of cancer, characterization of mutation and monitoring treatment response could greatly reduce the morbidity and mortality caused by cancer. Nevertheless, the extremely low amount of cell free nucleic acids makes the liquid biopsy a very challenging task. Herein, taking advantage of the pocked size, reliable quantitative results and simple operation of the pocket-sized personal glucose meter (PGM), we report an approach of circulating microRNA-21 (miR-21) detection with high precision and low cost. Via target-induced release of invertase from the DNA-invertase conjugate, which could convert sucrose into glucose, detection of miR-21 in serum was linked to PGM reading. Combined DNAzyme feedback amplification (DFA) program and highly efficient enzymatic turnover, an ultralow detection limit of 7 10-16 M for miR-21 was achieved using PGM as the reporter. The high sensitivity and selectivity of the proposed method meets the requirement of quantifying cell free nucleic acids in serum. In addition, this approach fills the shortage of quantitative RT-PCR and next-generation sequencing in quantifying miRNAs with short length, and greatly reduces the cost of detection. We believe the widely used personal diagnosis devices could hold an important place in the booming area of liquid biopsy.