Mo-BBS-02 - Enhanced IgE Aptasensing via Scaffold-mediated Oriented Immobilization of DNA Aptamers

Event

EUROSENSORS 2026
2026-09-06 - 2026-09-09
Zurich

Band

Poster

Chapter

Biosensors/-electronics And Biomimetic Sensors

Author(s)

Y. Chokki, A. Tanabe, I. Sasaki, M. Iijima - Tokyo Univ. Agric.,Tokyo (Japan), H. Nakano - Nagoya Univ.,Nagoya (Japan), S. Kuroda - Univ. of Osaka,Osaka (Japan)

Pages

338 - 339

DOI

10.5162/eurosensors2026/Mo-BBS-02

ISBN

978-3-910600-12-6

Price

free

Abstract

We developed a scaffold based on a single-chain Cro (scCro)-displaying bio-nanocapsule (scCroBNC) that enables the oriented immobilization of DNA aptamers, thereby achieving highly sensitive detection in an aptasensor system. Herein, scCro-BNC was applied to a quartz crystal microbalancebased aptasensor for the detection of immunoglobulin E, an important biomarker for allergy diagnosis. This approach improved the sensitivity by approximately 30-fold compared with direct immobilization, demonstrating that scCro-BNC is an effective scaffold for enhancing aptasensor performance.