Tu-BBS-15 - Optimization of ssDNA Immobilization for a CRISPR/Cas12a Electrochemical Biosensor for Friedreich’s Ataxia

Event

EUROSENSORS 2026
2026-09-06 - 2026-09-09
Zurich

Band

Poster

Chapter

Biosensors/-electronics And Biomimetic Sensors

Author(s)

L. V. D. Souza, A. M. Faria, T. Mazon - Centro de Tecnologia da Informação Renato Archer,Campinas (Brazil)

Pages

386 - 387

DOI

10.5162/eurosensors2026/Tu-BBS-15

ISBN

978-3-910600-12-6

Price

free

Abstract

An electrochemical biosensor based on CRISPR-Cas12a was developed by optimizing ssDNA immobilization on gold electrodes. Electrochemical impedance spectroscopy (EIS) was employed to characterize each modification step and the collateral cleavage activity. The optimized interface allowed Cas12a accessibility and resulted in a decrease in charge transfer resistance after collateral DNA cleavage. These results demonstrate that precise interface control is essential for developing CRISPR-based diagnostic platforms.