4.2.1 Bisphenol A Sensing Device Utilizing Antibody Modified Beads on a Microfluidic Disk
- Event
- 14th International Meeting on Chemical Sensors - IMCS 2012
2012-05-20 - 2012-05-23
Nürnberg/Nuremberg, Germany - Chapter
- 4.2 Biosensors IV (Systems)
- Author(s)
- I. Kubo, K. Moriwaki, T. Kanamatsu, S. Furutani - Faculty of engineering, Soka University (Japan)
- Pages
- 337 - 340
- DOI
- 10.5162/IMCS2012/4.2.1
- ISBN
- 978-3-9813484-2-2
- Price
- free
Abstract
Bisphenol A (BPA) is a major material of polycarbonate, which is used as a food container in our life and known as endocrine disruptor. In order to investigate the effect to health animal experiments are necessary. To determine the intake of BPA with food in animals, we need a sensing system of BPA with a small amount of biological sample in short reaction time. We have developed a disk-shaped microfluidic device for ELISA with 32 microchannels and chambers. In order to establish a rapid and sensitive assay system of BPA in biological sample, anti BPA antibody was immobilized on micro beads and introduced into microchambers on the microchannels on the device, in this study. Competitive immunoassay was performed using HRP conjugated BPA with a small amount of sample solution with in 20 min. BPA was determined on the microfluidic disk at the concentration range between 3.9- 250 ng ml^-1. BPA spiked rat serum was determined on the disk.