P1.9.1 Investigation of Water Penetration into Ceramides with Lateral Field Excitation Sensors
- 14th International Meeting on Chemical Sensors - IMCS 2012
2012-05-20 - 2012-05-23
- P1.9 Technology and Application
- J. Fochtmann, A. Kaestner, R. Lucklum - Otto-von-Guericke-University Magdeburg, Institute of Micro- and Sensor Systems (Germany), A. Schroeter, R. Neubert - Martin-Luther-University Halle, Institute for Pharmaceutics and Biopharmaceutics (Germany)
- 1151 - 1154
In this work we apply ceramide membranes using quartz crystal resonator sensors investigating water penetration into these membranes. The sensor response to pure Ceramide[AS] and Ceramide[AP] and mixtures with cholesterol immobilized onto the sensor surface and when immersed in deionized water is significantly different. After an annealing sequence to activate the ceramides, water immersion results in a resonance frequency shift of a classical Quartz Crystal Microbalance (QCM) primarily due to film mass increase caused by water absorption. Another sensor type, Lateral Field Excitation (LFE), shows qualitatively equal behavior: pure Ceramide[AS] or Ceramide[AP] do not display a diffusion related frequency shift whereas Ceramide[AS] mixed with cholesterol provides a significant change in resonance frequency and Q-factor.