P2.4.6 Fabrication of Microfluidic Chip-based Chemiluminescence Sensor by the Immobilization of Copper (II) on a MWCNT-Nafion Composite and Its Analytical Application

14th International Meeting on Chemical Sensors - IMCS 2012
2012-05-20 - 2012-05-23
Nürnberg/Nuremberg, Germany
P2.4 Nanostructured Sensors
M. Kamruzzaman, A. Alam, S. Lee - Department of Chemistry, Kyungpook National University (South Korea), Y. Kim - Research Institute of Advanced Energy Technology, Kyungpook National University (South Korea), G. Kim, T. Dang - School of Mechanical Engineering, Kyungpook National University (South Korea)
1513 - 1516


A novel chemiluminescence (CL) sensor has been fabricated by the immobilization of copper (II) on the functionalized MWCNT-nafion (NF) composite membrane using microfluidic chip. The functionalized MWCNT-NF composite was prepared by solution casting using 5 wt% ox-MWCNT in 5% NF solution. The metal ion (Cu2+) was then immobilized on to the MWCNT-NF composite which exhibits excellent catalytic activity towards the oxidation of luminol to determine ciprofloxacin (CIP), a fluoroquinolone (FQs) antibacterial agent as CNTs are the potential materials for catalyst supports. Thus, we developed a microfluidic chip based CL sensor to determine Cip based on a luminol-H2O2 CL assay. The microfluidic chip was fabricated by a soft-lithographic procedure using polydimethyl siloxane (PDMS). The fabricated PDMS microfluidic chip had three inlets with a 500 µm wide, 250 µm deep and 82 mm long microchannel. The prepared Cu2+ immobilized MWCNT-NF composite was characterized by SEM, EDS. CIP was sensed by the CL reaction between luminol and H2O2 in the presence of Cu2+ immobilized MWCNT-NF composite on the PDMS chip. The fabricated CL sensor on the PDMS chip provides sensitive determination of CIP with long lifetime without any special pretreatment of the sample. Due to the ease of operation and excellent catalytic properties of the proposed sensor, it may be a potential FQs sensor to determine FQs in real samples.