P2.4.7 Fabrication of Silver Nanoparticles Immobilzed Microfluidic Chip for Chemiluminescence based Analytical Application
- 14th International Meeting on Chemical Sensors - IMCS 2012
2012-05-20 - 2012-05-23
- P2.4 Nanostructured Sensors
- A. Alam, M. Kamruzzaman, S. Lee, K. Kim - Department of Chemistry, Kyungpook National University (South Korea), T. Dung - School of Mechanical Engineering, Kyungpook National University (South Korea), G. Kim, Y. Kim - Research Institute of Advanced Energy Technology, Kyungpook National University (South Korea)
- 1517 - 1520
Silver nanoparticles (Ag NPs) were successfully immobilized on polydimethylsiloxane (PDMS) surface in the microfluidic chip through surface modification using 3-aminopropyletriethoxy silane (APTES) followed by plasma treatment. The presence of Ag NPs was confirmed by UV-Vis and SEM-EDS spectra. The fabricated microfluidic chip was applied to determine the concentration of L-Cysteine (Cys) sensitively by luminol-H2O2 chemiluminescence (CL) system through its inhibition effect. The linearity of the CL signal from Luminol-Cys-H2O2 system in the fabricated sensor chip was observed in the range of 1.2 to 10.5 µM (r=0.9983) of Cys with a limit of detection (LOD) of 33 nM.