P2.2.3 Label free immune assay using terahertz chemical microscope

Event

14th International Meeting on Chemical Sensors - IMCS 2012
2012-05-20 - 2012-05-23
Nürnberg/Nuremberg, Germany

Chapter

P2.2 Resonant Sensors

Author(s)

T. Kiwa, A. Tenma, K. Sakai, K. Tsukada - Graduate School of Natural Science and Technology, Okayama University (Japan)

Pages

1410 - 1413

DOI

10.5162/IMCS2012/P2.2.3

ISBN

978-3-9813484-2-2

Price

free

Abstract

High sensitivity and throughput analysis of the interactions of proteins, label-free detection of antibodyantigen bindings are demanded. In group, a terahertz chemical microscope (TCM) has been proposed and developed to visualize the distribution of the antibody-antigen bindings on the silicon based sensing plate without any labels and/or markers on the antibody. As the first demonstration of the label free immune assay, the mouse immunoglobulin G (IgG) was immobilized on the half part of the sensing plate and the mouse anti-IgG was combined with the IgG. The distributions of the IgG and anti-IgG bindings on the sensing plate were clearly seen as the TCM images.