P2.2.3 Label free immune assay using terahertz chemical microscope
- 14th International Meeting on Chemical Sensors - IMCS 2012
2012-05-20 - 2012-05-23
- P2.2 Resonant Sensors
- T. Kiwa, A. Tenma, K. Sakai, K. Tsukada - Graduate School of Natural Science and Technology, Okayama University (Japan)
- 1410 - 1413
High sensitivity and throughput analysis of the interactions of proteins, label-free detection of antibodyantigen bindings are demanded. In group, a terahertz chemical microscope (TCM) has been proposed and developed to visualize the distribution of the antibody-antigen bindings on the silicon based sensing plate without any labels and/or markers on the antibody. As the first demonstration of the label free immune assay, the mouse immunoglobulin G (IgG) was immobilized on the half part of the sensing plate and the mouse anti-IgG was combined with the IgG. The distributions of the IgG and anti-IgG bindings on the sensing plate were clearly seen as the TCM images.