P1BS.11 - Development of SPR and fluorescence synchronous detection system
- Event
- 17th International Meeting on Chemical Sensors - IMCS 2018
2018-07-15 - 2018-07-19
Vienna, Austria - Chapter
- P-1 - Biosensors
- Author(s)
- L. Zhang, X. Chen, D. Cui, Y. Li, Y. Ren, C. Xu - State key laboratory of transducer technology, Institute of Electronics, Chinese Academy of Sciences, Beijing (China)
- Pages
- 495 - 496
- DOI
- 10.5162/IMCS2018/P1BS.11
- ISBN
- 978-3-9816876-9-9
- Price
- free
Abstract
Surface plasmon resonance (SPR) biosensors was an extremely sensitive optical technique to detect the changes in refractive index occurring at the sensor interface. SPR biosensors and fluorescence analysis were two kinds effective methods for real-time detection. The combination of these technology would improve the detection sensitivity of fluorescence quantitative and the specificity of SPR detection. We designed and developed a SPR and fluorescence synchronous detection system. SPR module was based on two kinds of modulation methods and fluorescence module could switch among 4 kinds of wavelength. SPR peak curves of fluorescent microspheres was collected by SPR angular scanning method.