P1BS.13 - Dual-signal Nanoswitchs for In Situ Intracellular Imaging of non-coding RNA
- Event
- 17th International Meeting on Chemical Sensors - IMCS 2018
2018-07-15 - 2018-07-19
Vienna, Austria - Chapter
- P-1 - Biosensors
- Author(s)
- J. Xu, X. Li, J. Wang, H. Chen - State Key Laboratory of Analytical Chemistry for Life Science and Collaborative Innovation Center of Chemistry for Life Sciences, School of Chemistry and Chemical Engineering, Nanjing University, Nanjing (China)
- Pages
- 499 - 500
- DOI
- 10.5162/IMCS2018/P1BS.13
- ISBN
- 978-3-9816876-9-9
- Price
- free
Abstract
With the fast development of imaging technology, nanomaterials owned excellent properties have been widely applied in the fields of imaging analysis. However, some shortcomings of imaging technology and nanomaterials, including low stability and sensitivity of probes, the "false positive" results, light bleaching and cell photo damage during in imaging analysis, still exist in the present analytical methods. To address these problems, we designed a novel type of optical nanoprobes owning bifunctional imaging properties, providing insight on fundamental understanding of the signal generation mechanism and transformation mechanism. The novel probes can be further combined with dark-field and laser confocal scanning microscope imaging technique, for establishing a dualimaging analysis strategy for intracellular non-coding RNA. Besides, we plan to extend the application scope of this method, study the intracellular multiple analytes simultaneous and dynamical imaging analysis in real-time, meanwhile imaging at molecule level, thereby provide powerful techniques for biochemical analysis.